Improved corrosion resistance and biocompatibility of a calcium phosphate coating on a magnesium alloy for orthopedic applications

Abstract

In this study a calcium phosphate (Ca–P) coating was fabricated on the surface of an AZ31 alloy by a chemical deposition process, and the in vitro and in vivo studies were carried out on a Ca–P-coated and uncoated AZ31 alloy to determine the effect of Ca–P coating on the corrosion behavior and biocompatibility of the AZ31 alloy. The morphology and composition of the Ca–P coating were characterized by scanning electron microscopy and energy dispersive spectroscopy. The corrosion behavior of the Ca-P coating was evaluated by a static immersion test and the effects of the Ca–P coating on biocompatibility were also investigated by in vitro cell experiments and in vivo animal experiments. The results indicated that the Ca–P coating reduced the in vitro and in vivo corrosion rates of the AZ31 alloy. Cell experiments showed significantly good adherence and high proliferation on the Ca–P-coated AZ31 alloy than those on the uncoated AZ31 alloy ( P < 0.05). The blood cell aggregation tests showed that the Ca–P-coated AZ31 alloy had decreased the blood cell aggregation compared to the uncoated AZ31 alloy. The animal experiments showed that the uncoated AZ31 alloy degraded more rapidly than the Ca–P-coated AZ31 alloy and the Ca–P coating provided significantly good biocompatibility, thus suggesting that the Ca–P coating not only slowed down the corrosion rate of the AZ31 alloy, but also improved its biocompatibility. Therefore, the Ca–P-coated AZ31 alloy can be considered as a promising biomaterial for orthopedic applications.