Potential Role of Agrimonia eupatoria L. Extract in Cell Protection Against Toxicity Induced by Bisphenol A

Abstract

The aim of this study is to reveal the potentially protective role of ethanolic extract of agrimony (Agrimonia eupatoria L.) against the cytotoxic effect of bisphenol A (BPA) in vitro, using an intestinal porcine epithelial cell line (IPEC-1). The cells were exposed to different concentrations of BPA: 12.5, 25, 50, 100, and 200 µg.ml–1 alone and in combination with agrimony extract (250 µg.ml–1). The proliferative cell response was monitored for 72 h by a xCELLigence system or real-time cell analyser (RTCA), recorded as the cell index (CI) and expressed as a proliferative activity (% PA) compared to the control cells without treatment. The metabolic activity was measured by a MTS colorimetric test, performed after 48 h of treatment with the tested substances. The cytotoxic effect on cells exposed to BPA alone, in comparison to the control cells without treatment, was observed in both assays (P < 0.0001). It was confirmed that BPA reduces both the metabolic activity and the proliferation of cells. After the cell treatment with agrimony, the metabolic activity had increased to reach over the control (101.52 %), while reducing the proliferation of the cells. The protective role of agrimony against cytotoxicity caused by BPA was observed after cell treatment with agrimony in combination with lower concentrations of BPA (12.5; 25 and 50 µg.ml–1). The slight improvement in the adherence was observed in cells treated with these combinations, in comparison to the cells treated with BPA alone. On the other hand, the metabolic activity was slightly improved in cells treated with a combination of agrimony and BPA at higher concentrations (50 a 100 µg.ml–1). This supported our assumption that agrimony can protect a model organism against cytotoxicity caused by BPA.

Keywords

• agrimony
• bisphenol a
• cytotoxicity
• intestinal cell line
• mts assay
• xcelligence