Antibiotics (Aug 2018)

Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae

  • Sumudu R Perera,
  • Ali Taheri,
  • Nurul H Khan,
  • Rajinder P Parti,
  • Stephanie Stefura,
  • Pauline Skiba,
  • Jason P Acker,
  • Irene Martin,
  • Anthony Kusalik,
  • Jo-Anne R Dillon

DOI
https://doi.org/10.3390/antibiotics7030070
Journal volume & issue
Vol. 7, no. 3
p. 70

Abstract

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Eleven primer pairs were developed for the identification of Neisseria gonorrhoeae. The sensitivity and specificity of these primers were evaluated by Real Time (RT)-PCR melt curve analyses with DNA from 145 N. gonorrhoeae isolates and 40 other Neisseria or non-Neisseria species. Three primer pairs were further evaluated in a hydrogel-based RT-PCR detection platform, using DNA extracted from 50 N. gonorrhoeae cultures. We observed 100% sensitivity and specificity in the hydrogel assay, confirming its potential as a point-of-care test (POCT) for N. gonorrhoeae diagnosis.

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