Genetic screens in isogenic mammalian cell lines without single cell cloning

Peter C. DeWeirdt; Annabel K. Sangree; Ruth E. Hanna; Kendall R. Sanson; Mudra Hegde; Christine Strand; Nicole S. Persky; John G. Doench

doi:10.1038/s41467-020-14620-6

Nature Communications (Feb 2020)

Genetic screens in isogenic mammalian cell lines without single cell cloning

Peter C. DeWeirdt,
Annabel K. Sangree,
Ruth E. Hanna,
Kendall R. Sanson,
Mudra Hegde,
Christine Strand,
Nicole S. Persky,
John G. Doench

Affiliations

Peter C. DeWeirdt: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Annabel K. Sangree: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Ruth E. Hanna: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Kendall R. Sanson: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Mudra Hegde: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Christine Strand: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Nicole S. Persky: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
John G. Doench: Genetic Perturbation Platform, Broad Institute of MIT and Harvard

DOI: https://doi.org/10.1038/s41467-020-14620-6
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 15

Abstract

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Isogenic pairs of cell lines are powerful tools but time-consuming to generate. Here the authors conduct genome-wide genetic interactions screens of ‘anchor’ genes with SaCas9 and SpCas9.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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