The objective of this research was to determine the relationship between blood cholesterol and acute ischemic stroke (AIS) among type-2 diabetic patients (TDP). A case-control study was conducted among TDP attending the Bhuddasothorn hospital in Chachoengsao, with 100 cases and 300 controls in 2013-2016. Cases and controls were matched by gender, age, residential area, and duration of attendance. Data were collected with a questionnaire comprising 2 parts: part 1 demographic characteristics, and part 2 clinical and health data. The obtained data were analyzed using descriptive statistics and analytic statistics with a computerized statistical package. Multivariable conditional logistic regression analysis, controlling for possible confounding factors, revealed that a total cholesterol of 241-280 and > 280 mg/dl increased the risk of AIS by a factor of 4.3 and 7.7 times, respectively (OR=4.3, 95%CI=1.4-13.7; OR=7.7, 95%CI =1.1-57.5). A surveillance system monitoring blood cholesterol among risk groups should be conducted in cooperation with information regarding cholesterol control and stroke prevention among TDP as an essential measure to reduce AIS risk.