Application of <i>Ligilactobacillus salivarius</i> SP36, a Strain Isolated from an Old Cheese Seal, as an Adjunct Culture in Cheesemaking
Ramón Arias,
Claudio Alba,
Javier Calzada,
Lorena Jiménez,
Leónides Fernández,
Marta Ávila,
Mario Roman,
Juan Roman,
Juan M. Rodríguez,
Sonia Garde
Affiliations
Ramón Arias
Centro Regional de Selección y Reproducción Animal (CERSYRA), Instituto Regional de Investigación y Desarrollo Agroalimentario y Forestal de la Junta de Comunidades de Castilla-La Mancha (IRIAF), CERSYRA Valdepeñas, 13300 Ciudad Real, Spain
Claudio Alba
Department of Nutrition and Food Science, Complutense University of Madrid, 28040 Madrid, Spain
Javier Calzada
Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Carretera de La Coruña km 7, 28040 Madrid, Spain
Lorena Jiménez
Centro Regional de Selección y Reproducción Animal (CERSYRA), Instituto Regional de Investigación y Desarrollo Agroalimentario y Forestal de la Junta de Comunidades de Castilla-La Mancha (IRIAF), CERSYRA Valdepeñas, 13300 Ciudad Real, Spain
Leónides Fernández
Department of Galenic Pharmacy and Food Technology, Complutense University of Madrid, 28040 Madrid, Spain
Marta Ávila
Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Carretera de La Coruña km 7, 28040 Madrid, Spain
Mario Roman
QUALIAM s.l., Velazquez 119, 28003 Madrid, Spain
Juan Roman
QUALIAM s.l., Velazquez 119, 28003 Madrid, Spain
Juan M. Rodríguez
Department of Nutrition and Food Science, Complutense University of Madrid, 28040 Madrid, Spain
Sonia Garde
Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Carretera de La Coruña km 7, 28040 Madrid, Spain
Adjunct cultures originating from artisanal cheese environments may play an important role in recreating and developing traditional cheese flavours, thanks to their enzymatic activities, involved in different metabolic pathways that occur during cheese ripening. In this work, Ligilactobacillus salivarius SP36, a strain isolated from an old cheese seal, was added as an adjunct culture to the cheese’s raw milk, and its effect on the microbiological, physical–chemical and sensory characteristics of the cheese was studied. The use of L. salivarius SP36 in cheese manufacturing had no significant (p > 0.05) effect on the cheese microbiota, gross composition (fat percentage, protein, total solids, moisture and NaCl concentration), colour or texture of the cheese. However, L. salivarius SP36 increased (p L. salivarius SP36 received higher scores (p L. salivarius SP36 proved to be a good candidate as an adjunct culture for cheesemaking, since it improved the cheese flavour by making it more intense and recovering typical sensorial notes of traditional cheeses.