Status of antioxidant enzymes in different body organs of male buffaloes

Abstract

Eighteen tissue samples each from five organs i.e. liver, lung, heart, kidney and skeletal muscle were collected immediately after slaughter of adult male buffaloes and taken to laboratory on the same day in cold normal saline solution. After homogenization of tissues, they were centrifuged at 3000 rpm for 30 minutes at 4°C. The supernatant collected from individual homogenized tissues were used for antioxidant enzymes viz. Superoxide dismutase (SOD), Catalase and Thiobarbituric acid reactive substance (TBARS). The levels of SOD and catalase were maximum in heart tissues and minimum in kidney tissues. The activity of TBARS was highest in liver tissues and lowest in skeletal muscles. The activity of SOD and catalase was significantly (P<0.01) different in the different tissues. The levels of catalase and TBARS varied significantly (P<0.01) at different intervals of sample collection. Catalase and SOD activities were positively correlated (P<0.01) in cardiac muscle. Similarly, TBARS and catalase also showed significant (P<0.01) positive correlation in different tissues. The data indicates, the varied levels of different antioxidant enzymes in various body tissues mainly due to different rate of metabolism and climate conditions therefore the information generated may be useful for assessing the stress levels in buffaloes.

Keywords

• Antioxidant, Body tissue, Buffalo