Light: Advanced Manufacturing (Feb 2022)

The application of digital holography for accurate three-dimensional localisation of mosquito-bednet interaction

  • Matthew L Hall,
  • Katherine Gleave,
  • Angela Hughes,
  • Philip J McCall,
  • Catherine E Towers,
  • David P Towers

DOI
https://doi.org/10.37188/lam.2022.020
Journal volume & issue
Vol. 3, no. 1
pp. 1 – 15

Abstract

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Understanding mosquito interaction with long-lasting insecticidal bednets is crucial in the development of more effective intervention methods to protect humans from malaria transmission. As such, a 240 × 240 × 1000 mm laboratory setup for the in-line recording of digital holograms and subsequent in-focus reconstruction and 3D localisation of mosquitoes is presented. Simple bednet background removal methods are used to accurately localise a mosquito obscured by a bednet in 3D coordinates. Simulations and physical data demonstrate that this method is suitable for mosquitoes positioned 3−1000 mm behind a bednet. A novel post-processing technique, involving a cascade-correlation of a Tamura of Intensity focus metric extracted from digitally reconstructed scenes, accurately localises mosquitoes positioned 35−100 mm behind a bednet from a single digital hologram. The result of this study is a scalable digital holographic methodology to examine mosquito-bednet interaction in 3D at a level of accuracy previously only seen in 2D imaging of mosquitoes in a much smaller volume.

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