Simultaneous electrophysiology and optogenetic perturbation of the same neurons in chronically implanted animals using μLED silicon probes

Nathaniel R. Kinsky; Mihály Vöröslakos; Jose Roberto Lopez Ruiz; Laurel Watkins de Jong; Nathan Slager; Sam McKenzie; Euisik Yoon; Kamran Diba

STAR Protocols (Dec 2023)

Simultaneous electrophysiology and optogenetic perturbation of the same neurons in chronically implanted animals using μLED silicon probes

Nathaniel R. Kinsky,
Mihály Vöröslakos,
Jose Roberto Lopez Ruiz,
Laurel Watkins de Jong,
Nathan Slager,
Sam McKenzie,
Euisik Yoon,
Kamran Diba

Affiliations

Nathaniel R. Kinsky: Department of Anesthesiology and Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Corresponding author
Mihály Vöröslakos: Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA; Neuroscience Institute, Langone Medical Center, New York University, New York, NY 10016, USA
Jose Roberto Lopez Ruiz: Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
Laurel Watkins de Jong: Department of Anesthesiology and Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Nathan Slager: Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
Sam McKenzie: Department of Neuroscience, University of New Mexico, Albuquerque, NM 87131, USA
Euisik Yoon: Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Center for Nanomedicine, Institute for Basic Science (IBS) and Graduate Program of Nano Biomedical Engineering (Nano BME), Advanced Science Institute, Yonsei University, Seoul 03722, South Korea
Kamran Diba: Department of Anesthesiology and Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Corresponding author

Journal volume & issue: Vol. 4, no. 4
p. 102570

Abstract

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Summary: Micro-light-emitting-diode (μLED) silicon probes feature independently controllable miniature light-emitting-diodes (LEDs) embedded at several positions in each shank of a multi-shank probe, enabling temporally and spatially precise optogenetic neural circuit interrogation. Here, we present a protocol for performing causal and reproducible neural circuit manipulations in chronically implanted, freely moving animals. We describe steps for introducing optogenetic constructs, preparing and implanting a μLED probe, performing simultaneous in vivo electrophysiology with focal optogenetic perturbation, and recovering a probe following termination of an experiment.For complete details on the use and execution of this protocol, please refer to Watkins de Jong et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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