A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons

Anastasiya Sybirna; Walfred W. C. Tang; Merrick Pierson Smela; Sabine Dietmann; Wolfram H. Gruhn; Ran Brosh; M. Azim Surani

doi:10.1038/s41467-020-15042-0

Nature Communications (Mar 2020)

A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons

Anastasiya Sybirna,
Walfred W. C. Tang,
Merrick Pierson Smela,
Sabine Dietmann,
Wolfram H. Gruhn,
Ran Brosh,
M. Azim Surani

Affiliations

Anastasiya Sybirna: Wellcome Trust/Cancer Research UK Gurdon Institute, Henry Wellcome Building of Cancer and Developmental Biology
Walfred W. C. Tang: Wellcome Trust/Cancer Research UK Gurdon Institute, Henry Wellcome Building of Cancer and Developmental Biology
Merrick Pierson Smela: Wellcome Trust/Cancer Research UK Gurdon Institute, Henry Wellcome Building of Cancer and Developmental Biology
Sabine Dietmann: Wellcome Trust/Medical Research Council Cambridge Stem Cell Institute, University of Cambridge
Wolfram H. Gruhn: Wellcome Trust/Cancer Research UK Gurdon Institute, Henry Wellcome Building of Cancer and Developmental Biology
Ran Brosh: Institute for Systems Genetics, NYU Langone Health
M. Azim Surani: Wellcome Trust/Cancer Research UK Gurdon Institute, Henry Wellcome Building of Cancer and Developmental Biology

DOI: https://doi.org/10.1038/s41467-020-15042-0
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 18

Abstract

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PRDM14 is a critical transcription factor for mouse primordial germ cell specification, but its role in human remains unclear. Here, PRDM14 protein depletion using auxin-inducible degron uncovers a critical role for human germ cell specification, but regulation of a different set of target genes than in mouse.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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