Морфологія (Jan 2013)
Submicroscopic сhanges in the thyroid gland of rats after the immunosuppression
Abstract
Background. A significant number of patients receive immunosuppressive drugs for the treatment of autoimmune, allergic diseases, organ and tissue transplantation. In this regard, the study of reactivity of the thyroid gland during immunosuppression caused by the introduction of cyclophosphamide is of current interest. Objective. To investigate the submicroscopic changes in the thyroid gland at the single administration of cyclophosphan in high doses. Methods. The research was conducted on 24 male rats with an initial body weight of 180±10, which were divided into 4 groups. The animals of 1st and the 2nd groups received cyclophosphan in a dose of 200 mg/kg body weight. The 3rd and the 4th groups included control (intact) animals. The material was taken on 3rd and 30th days after the injection of cyclophosphan in accordance with the immune response phases. Results. The conducted electron microscopy research revealed significant changes of thyrocyte organelles and thyroid gland stroma during the short term of observation (3 days). After 30 days of cyclophosphan injection reactive changes directed at the normalization of the gland structure took place. Conclusion. It is planned further to investigate the ultrastructure of the thyroid gland of animals after correction of cyclophosphan-inducted immunosuppression with imunofan.
