Molecular diagnosis with the corresponding clinical symptoms of canine distemper virus infection in javan leopard (Panthera pardus ssp. melas)
Dede Aulia Rahman,
Uus Saepuloh,
Yanto Santosa,
Huda Shalahudin Darusman,
Irene Margareth Romaria Pinondang,
Andy Sean Kindangen,
Amira Putri Pertiwi,
Lana Sari,
Andi Irawan,
Keni Sultan,
Puji Rianti
Affiliations
Dede Aulia Rahman
Department of Forest Resources Conservation and Ecotourism, Faculty of Forestry and Environment, IPB University (Bogor Agricultural University), Bogor, Indonesia; Primate Research Center, Institute of Research and Community Service, IPB University (Bogor Agricultural University), Bogor, Indonesia; Javan Leopard Conservation Forum, Bogor, Indonesia; Corresponding author.
Uus Saepuloh
Primate Research Center, Institute of Research and Community Service, IPB University (Bogor Agricultural University), Bogor, Indonesia
Yanto Santosa
Department of Forest Resources Conservation and Ecotourism, Faculty of Forestry and Environment, IPB University (Bogor Agricultural University), Bogor, Indonesia
Huda Shalahudin Darusman
Primate Research Center, Institute of Research and Community Service, IPB University (Bogor Agricultural University), Bogor, Indonesia; Faculty of Veterinary Medicine, IPB University (Bogor Agricultural University), Bogor, Indonesia
Irene Margareth Romaria Pinondang
Javan Leopard Conservation Forum, Bogor, Indonesia; Durrel Institute of Conservation and Ecology, School of Anthropology and Conservation, University of Kent, Canterbury, United Kingdom
Andy Sean Kindangen
Javan Leopard Conservation Forum, Bogor, Indonesia; Animal Sanctuary Trust Indonesia, Bogor, Indonesia
Amira Putri Pertiwi
Animal Sanctuary Trust Indonesia, Bogor, Indonesia
Lana Sari
Center for Conservation of Natural Resources of West Java, Ministry of Environmental and Forestry, Bandung, Indonesia
Andi Irawan
Center for Conservation of Natural Resources of West Java, Ministry of Environmental and Forestry, Bandung, Indonesia
Keni Sultan
Javan Leopard Conservation Forum, Bogor, Indonesia; Taman Safari Indonesia, Bogor, Indonesia
Puji Rianti
Primate Research Center, Institute of Research and Community Service, IPB University (Bogor Agricultural University), Bogor, Indonesia; Department of Biology, Faculty of Mathematics and Natural Sciences, IPB University (Bogor Agricultural University), Bogor, Indonesia
Diseases are one of the possible threats to the conservation of wild cat populations. One disease that has been reported to infect and cause death, including in various wildlife, is the canine distemper virus (CDV). Here, we report the first case of CDV in an adolescent melanistic Javan female leopard in Indonesia. We combined the clinical report with the Reverse Transcription PCR (RT-PCR) analyses on the faecal and brain samples of partial nucleoprotein (CDV-N) and hemagglutinin (CDV-H) genes. We also performed analyses of urine, haematology, and blood chemistry. The CDV-H nucleotide sequence confirmed the CDV infection in the female leopard and was clustered to the CDV's Asia 1 genotype. This finding opens an investigating window to analyse the pathogen transmission between animals in wildlife, particularly to support the management of conservation in natural habitats in Indonesia.
