Genetics and Molecular Biology (Jan 2008)

DNA methylation analysis of the tumor suppressor gene CDKN2B in Brazilian leukemia patients

  • Patrícia Santos Pereira Lima,
  • Greice Andreoti Molffeta,
  • Amélia Góes de Araujo,
  • Marco Antônio Zago,
  • Wilson Araújo da Silva Jr.

DOI
https://doi.org/10.1590/S1415-47572008000400005
Journal volume & issue
Vol. 31, no. 3
pp. 632 – 638

Abstract

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The aim of this work was to evaluate the methylation profile of the p15 (CDKN2B) gene in Brazilian patients with leukemia and to correlate the CDKN2B gene expression with the percentage of methylated CpG dinucleotides in its promoter region. Thirty-one samples from six patients with acute lymphocytic leukemia (ALL), four with chronic myeloid leukemia (CML), and 21 with acute myeloid leukemia (AML) were evaluated by MSP (Methylation-Specific PCR). The CDKN2B gene was found to be methylated in four (67%) of the six ALL samples and in 16 (76%) of the 21 AML samples, but in none of the four CML samples analyzed. We observed a correlation between the CDKN2B mRNA expression (RT-PCR) and the percentage of methylated CpG dinucleotides. Therefore, this study in Brazilian patients confirms that the CDKN2B gene is methylated in the majority of leukemia patients.

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