A proper placental sampling for syncytin-1 analysis
Petra Priščáková,
Miroslav Korbeľ,
Zuzana Nižňanská,
Katarína Letkovská,
Katarína Sušienková,
Vanda Repiská,
Daniel Böhmer,
Helena Gbelcová
Affiliations
Petra Priščáková
1Institute of Medical Biology, Genetics & Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava & University Hospital Bratislava, Sasinkova 4, 81108 Bratislava, Slovak Republic
Miroslav Korbeľ
2First Department of Gynaecology & Obstetrics, Faculty of Medicine, Comenius University in Bratislava & University Hospital Bratislava, Antolská 11, 85107 Bratislava, Slovak Republic
Zuzana Nižňanská
2First Department of Gynaecology & Obstetrics, Faculty of Medicine, Comenius University in Bratislava & University Hospital Bratislava, Antolská 11, 85107 Bratislava, Slovak Republic
Katarína Letkovská
3Institute of Pathological Anatomy, Faculty of Medicine, Comenius University in Bratislava & University Hospital Bratislava, Sasinkova 4, 81108 Bratislava, Slovak Republic
Katarína Sušienková
1Institute of Medical Biology, Genetics & Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava & University Hospital Bratislava, Sasinkova 4, 81108 Bratislava, Slovak Republic
Vanda Repiská
1Institute of Medical Biology, Genetics & Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava & University Hospital Bratislava, Sasinkova 4, 81108 Bratislava, Slovak Republic
Daniel Böhmer
1Institute of Medical Biology, Genetics & Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava & University Hospital Bratislava, Sasinkova 4, 81108 Bratislava, Slovak Republic
Helena Gbelcová
1Institute of Medical Biology, Genetics & Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava & University Hospital Bratislava, Sasinkova 4, 81108 Bratislava, Slovak Republic
Syncytin-1 (gene ERVW-1) has been proposed as a marker of pre-eclampsia and malfunctions in placental development. Placenta is heterogeneous tissue, hence the method of biopsy can significantly affect the outcome of analyses. A total of 44 placentae were analyzed by taking 3–30 samples from each. Relative levels of ERVW-1 expression in the placental biopsies were characterized by RT-qPCR. Evaluation of ten biopsies from one placenta individually (not pooling them) is recommended due to the high variability of expression. No significant correlation was found between biopsy localization and level of ERVW-1 expression; therefore, random sampling is recommended. A long cut from the umbilical cord to the edge of the placenta is a convenient approach to placental sampling.
