Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) 2-Hydroxyisoflavanone Dehydratasedase Gene

Abstract

The complete mRNA sequence of one tobacco (nicotiana tabacum) gene—2-hydroxyisoflavanone dehydratasedase, was amplified using the rapid amplification of cDNA ends methods based on one tobacco EST. The full-length tobacco 2-hydroxyisoflavanone dehydratasedase gene mRNA was 1,278bp containing a 966 bp open reading frame, which encodes a protein of 321 amino acids. Sequence analysis revealed that the 2-hydroxyisoflavanone dehydratasedase of tobacco shares high homology with the 2-hydroxyisoflavanone dehydratasedase of nicotiana tomentosiformis(99%), capsicum annuum(78%), potato(75%), lycopersicon pennellii(73%) and lycopersicon esculentum(72%). BLAST analysis within the tobacco high throughout genomic sequences database revealed that this gene has no intron and is a single exon gene. Results also showed that tobacco 2-hydroxyisoflavanone dehydratasedase gene has a closer genetic relationship with the 2-hydroxyisoflavanone dehydratasedase gene of nicotiana tomentosiformis. Tissue expression profile analysis revealed that the tobacco 2-hydroxyisoflavanone dehydratasedase gene was highly expressed in leaf and flower, but moderately expressed in root and stem. Our experiment established the foundation for further research on this tobacco gene.