Effect of microRNA‐181b on the biological characteristics and clinical drug resistance of small‐cell lung cancer by targeting angiotensin converting enzyme 2

Abstract

Abstract Objective To clarify the effect of miR‐181b on the biological function of small‐cell lung cancer (SCLC) and explore the effect of clinical resistance on SCLC. Methods Blood samples were collected from 30 SCLC patients and 30 non‐SCLC patients in our department from 2017 to 2019 to detect the expression level of miR‐181b.The expression level of miR‐181b was detected in SCLC cells by RT‐PCR, and screening of downstream target genes by gene chip, verification with luciferase, and Western blotting. In addition, collect the general data of 30 SCLC patients and 30 non‐SCLC patients (control group), the patients were diagnosed by pathology and undergoing EC protocol in the Department of Thoracic Surgery and Oncology of our hospital to detect the expression level of mir‐181b in different periods. Furthermore, in the SCLC cell line, EC chemotherapy was administered to detect the sensitivity of drug resistance and nondrug resistance. Results miR‐181b in SCLC patients was lower than in normal people as well as the drug‐sensitive cell line. ACE2 was verified as a downstream target of miR‐181b by gene chip screening. First‐line chemotherapy can promote the recovery of miR‐181b, but cannot repair to normal levels. miR‐181b can enhance the drug sensitivity of SCLC drug‐resistant cells. Conclusion miR‐181b directly targets ACE2 to affect the biological characteristics of SCLC. The expression level of miR‐181b is highly related to the drug resistance of SCLC, which suggests that miR‐181b could be a potential biomarker candidate for treatment efficacy of SCLC.

Keywords

• ACE2
• drug resistance
• microRNA (miR)‐181b
• small cell lung cancer