An EMS mutant library for cucumber

Chen CHEN; Qing-zhi CUI; San-wen HUANG; Shen-hao WANG; Xiao-hong LIU; Xiang-yang LU; Hui-ming CHEN; Yun TIAN

Journal of Integrative Agriculture (Jul 2018)

An EMS mutant library for cucumber

Chen CHEN,
Qing-zhi CUI,
San-wen HUANG,
Shen-hao WANG,
Xiao-hong LIU,
Xiang-yang LU,
Hui-ming CHEN,
Yun TIAN

Affiliations

Chen CHEN: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P.R. China; Hunan Vegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, P.R. China; Key Laboratory for Agricultural Biochemistry and Biotransformation of Hunan Provincial University, Changsha 410128, P.R. China
Qing-zhi CUI: Hunan Vegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, P.R. China
San-wen HUANG: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture/Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China
Shen-hao WANG: Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture/Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China
Xiao-hong LIU: Hunan Vegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, P.R. China
Xiang-yang LU: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P.R. China; Key Laboratory for Agricultural Biochemistry and Biotransformation of Hunan Provincial University, Changsha 410128, P.R. China; Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, P.R. China
Hui-ming CHEN: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P.R. China; Hunan Vegetable Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, P.R. China; Key Laboratory for Agricultural Biochemistry and Biotransformation of Hunan Provincial University, Changsha 410128, P.R. China; Correspondence CHEN Hui-ming
Yun TIAN: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, P.R. China; Key Laboratory for Agricultural Biochemistry and Biotransformation of Hunan Provincial University, Changsha 410128, P.R. China; Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, Changsha 410128, P.R. China

Journal volume & issue: Vol. 17, no. 7
pp. 1612 – 1619

Abstract

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Cucumber is an important vegetable crop and a model crop for the study of sex expression in plants. However, the genomic resources and tools for functional genomics studies in cucumber are still limited. In this paper, we conducted ethyl methyl sulfone (EMS) mutagenesis in the northern China ecotype cucumber inbred line 406 to construct a mutant library. We optimized the conditions of EMS mutagenesis on inbred line 406 which included treatment of seeds at 1.5% EMS for 12 h. We obtained a number of mutant lines showing inheritable morphological changes in plant architecture, leaves, floral organs, fruits and other traits through M1, M2 and M3 generations. The F2 segregating populations were constructed and analyzed. We found that a short fruit mutant and a yellow-green fruit peel mutant were both under the control of a single recessive gene, respectively. These results provide valuable germplasm resources for the improvement of cucumber genetics and functional genomic research.

Published in Journal of Integrative Agriculture

ISSN: 2095-3119 (Print); 2352-3425 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.journals.elsevier.com/journal-of-integrative-agriculture

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