Optimization of culture conditions for laccase production from Abortiporus biennis in a pilot-scale bioreactor

Abstract

The optimal culture conditions of laccase productions in submerged culture by Abortiporus biennis in a pilotscale bioreactor (300 L) were determined using the rotating simplex method. The optimal culture conditions were C/N ratio 25, agitation speed 200 rpm, aeration rate 1.5. In the optimal culture medium, the maximum laccase production was 1361 U/L in the pilot-scale bioreactor. Further investigation of the mycelial pellets and culture broth during the fermentation period revealed that the mean diameter and compactness of the pellet and viscosity of the broth were significantly positively correlated with laccase production. The results demonstrated the feasibility of A. biennis fermentation for largescale production of laccase.

Keywords

• Mycelia
• Abortiporus biennis
• fermentation
• morphology
• rotating simplex method