IL6 supports long-term expansion of hepatocytes in vitro

Ren Guo; Mengmeng Jiang; Gang Wang; Bing Li; Xiaohui Jia; Yan Ai; Shanshan Chen; Peilan Tang; Aijie Liu; Qianting Yuan; Xin Xie

doi:10.1038/s41467-022-35167-8

Nature Communications (Nov 2022)

IL6 supports long-term expansion of hepatocytes in vitro

Ren Guo,
Mengmeng Jiang,
Gang Wang,
Bing Li,
Xiaohui Jia,
Yan Ai,
Shanshan Chen,
Peilan Tang,
Aijie Liu,
Qianting Yuan,
Xin Xie

Affiliations

Ren Guo: State Key Laboratory of Drug Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Mengmeng Jiang: State Key Laboratory of Drug Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Gang Wang: Department of Pharmaceutics, School of Pharmacy, Fudan University
Bing Li: State Key Laboratory of Drug Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Xiaohui Jia: State Key Laboratory of Drug Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Yan Ai: State Key Laboratory of Drug Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Shanshan Chen: State Key Laboratory of Drug Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Peilan Tang: State Key Laboratory of Drug Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Aijie Liu: State Key Laboratory of Drug Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Qianting Yuan: State Key Laboratory of Drug Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Xin Xie: State Key Laboratory of Drug Research, National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences

DOI: https://doi.org/10.1038/s41467-022-35167-8
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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Hepatocytes are very difficult to expand in vitro. Here the authors discover that IL6 promotes long-term expansion (>30 passages) of primary mouse hepatocytes in vitro by converting the cells into hepatic progenitor cells, which maintain full capacity of differentiation into hepatocytes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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