Fourier Transform Infrared Spectroscopy combined with chemometrics for quality control of Curcuma aeruginosa rhizomes: An essential oil analysis

Abstract

Rhizome of Curcuma aeruginosa is a traditional herb utilized in many nations. The compound content in C. aeruginosa rhizomes can vary depending on their geographical origin. This study aims to determine the chemical fingerprints for quality control and identify the active ingredients in C. aeruginosa essential oil (CAEO) based on multiple geographic origins. Chemical fingerprints of CAEO from five different geographic origins have been determined using Fourier Transform Infrared Spectroscopy (FTIR). Principal component analysis and partial least squares discriminant analysis were utilized to examine the chemical data of CAEO utilizing pattern recognition techniques. A total of 52 fluctuating compounds were identified in gas chromatography-mass spectra instrumentation, and the highest essential oil result was the 1,8-cineole compound content. According to the results from the FTIR analysis, CAEO samples were successfully grouped based on their geographical origins. The experimental results showed that the wave numbers 1489.0715, 1475.3892, 1520.7538, and 1653.0743 per cm were used as markers to identify compounds in the CAEO based on the differences in the place of growth and quality control. This study showed that chemometric methods combined with FTIR-based fingerprinting could offer a dependable platform for verifying quality control and authentication.

Keywords

• curcuma aeruginosa; chemometric; essential oil; fourier transform infrared spectroscopy; gas chromatography-mass spectra