Inoculum for the cultivation of Methylosinus trichosporium

Abstract

In this study, the effect of the amount of introduced Methylosinus trichosporium culture inoculum on the specific growth rate was evaluated in order to improve the cultivation process. The variation of the studied factor allowed us to establish the dependence and determine the optimal dose of the inoculum for the culture in question. The prepared samples containing a nutrient medium with different inoculate content were cultured for 48 hours, during which the trend of changes in the optical density of the samples was studied. Based on the fact that the optical density is directly proportional to the concentration of microorganisms in the samples, their specific growth rate was calculated. The study showed that the culture of Methylosinus trichosporium demonstrates a stable growth rate in the range from 3 to 24 hours of cultivation, at such an inoculation dose at which the optical density of the nutrient medium with the introduced inoculum is 0.07-0.55. The data obtained provide tools for regulating the process of cultivation of Methylosinus trichosporium methanotrophs.