Antioxidative Effects of Standardized <i>Aronia melanocarpa</i> Extract on Reproductive and Metabolic Disturbances in a Rat Model of Polycystic Ovary Syndrome
Jovan Rudic,
Vladimir Jakovljevic,
Nikola Jovic,
Maja Nikolic,
Jasmina Sretenovic,
Slobodanka Mitrovic,
Sergey Bolevich,
Stefani Bolevich,
Miroslav Mitrovic,
Sasa Raicevic,
Kristina Andric,
Andjela Dimkic Milenkovic,
Dejana Rakic,
Jovana Joksimovic Jovic
Affiliations
Jovan Rudic
Clinic for Gynecology and Obstetrics Narodni Front, 11000 Belgrade, Serbia
Vladimir Jakovljevic
Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia
Nikola Jovic
Department of Gynecology and Obstetrics, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia
Maja Nikolic
Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia
Jasmina Sretenovic
Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia
Slobodanka Mitrovic
University Clinical Center Kragujevac, Zmaj Jovina 30, 34000 Kragujevac, Serbia
Sergey Bolevich
Department of Human Pathology, I.M. Sechenov First Moscow State Medical University, 119991 Moscow, Russia
Stefani Bolevich
Department of Pathophysiology, I.M. Sechenov First Moscow State Medical University, 119991 Moscow, Russia
Miroslav Mitrovic
Pharmanova, St. Miloja Djaka No 1, 11010 Belgrade, Serbia
Sasa Raicevic
Medical Faculty, University of Montenegro, 81000 Podgorica, Montenegro
Kristina Andric
University Clinical Center Kragujevac, Zmaj Jovina 30, 34000 Kragujevac, Serbia
Andjela Dimkic Milenkovic
Clinic for Burns, Plastic and Reconstructive Surgery, University Clinical Center of Serbia, Zvecanska 9, 11000 Belgrade, Serbia
Dejana Rakic
Department of Gynecology and Obstetrics, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia
Jovana Joksimovic Jovic
Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia
Polycystic ovary syndrome (PCOS) represents the most common endocrinopathy among childbearing-age women, with oxidative stress (OS) underlying its etiopathogenesis. Metformin (MET) represents a frequently used agent in PCOS. However, weak results encourage alternative treatments. We aimed to investigate isolated and synergistic effects of Standardized Aronia melanocarpa extract (SEA) and MET for alleviating reproductive and metabolic PCOS abnormalities. PCOS induction was followed by 28-day treatment with MET, SAE, or MET + SEA. Bodyweight (BW), cyclicity, histological, and ultrasonographical ovarian analyses were performed. Hormonal, glycemic, and lipid profiles were accessed, as well as systemic and ovarian oxidative status; BW, cyclicity, ovarian histomorphology, ovarian volume, testosterone and progesterone levels, as well as LDL, triglycerides, and total cholesterol levels were aggravated after PCOS-induction and improved after MET, SEA, and MET + SEA treatment. MET + SEA had the greatest impact on glycoregulation. Alterations in OS parameters (TBARS, O2−, H2O2, catalase, superoxide dismutase, and reduced glutathione) could be responsible for observed differences; (4) Conclusions: Our findings confirmed that SAE alone or along with MET was capable of ameliorating reproductive and metabolic disturbances in the PCOS rat model, with the restoration of OS parameters. SAE alone did not alter the protective effects of MET in PCOS.
