Nature Communications (May 2016)
Mechanical slowing-down of cytoplasmic diffusion allows in vivo counting of proteins in individual cells
Abstract
Several proteins are expressed at too low abundance in the Escherichia coli (E. coli) proteome to be detected by standard methods. Here, the authors create a microfluidics-based platform enabling single-molecule counting of low-abundance proteins by mechanically slowing-down their diffusion in live E. coli.