MORPHOGENESIS OF SALT RESISTANT CELL LINES OF CAMELINA SATIVA

Abstract

The creation and introduction into production of adaptive varieties is a prerequisite for efficient crop management in areas with adverse agro-climatic conditions. Nowadays, it is advisable to include a biotechnological link in the breeding process of creating resistant varieties. The use of in vitro culture can more accurately investigate the effect of a stress factor on the organism, control the physical and trophic parameters of the cultivation of the material, simulate the effect of selective agent on the biological object. However, there are some difficulties with the use of cell selection, the main one being the difficulty of obtaining regenerating plants due to tissue loss of morphogenesis capacity. The aim of the study was to analyze the effect of the genotype and conditions of regeneration while maintaining the morphogenic potential of salt resistant cell lines of camelina sativa and to obtaine regenerating plants. In the course of the stepped cell selection, the callus lines of the camelina sativa varieties of Stepovy 1, Peremoha, Yevro 12 and Klondaik with maximum resistance to salt stress were succeeded (maximum NaCl concentration in the nutrient medium was 1.5 %). To obtain regenerant plants, the created biomaterials were transferred to regenerative nutrient media according to Murasige-Skuga prescription, modified with increased cytokinin concentrations (1.0 mg/l 6-BAP). Regeneration was performed in the presence of a stress agent and in the conditions of the control variant. Chloride salinization exerts strong stress on the tissue culture of camelina sativa. Along with the inhibition of proliferation, a decrease in the ability to morphogenesis of created cell lines was observed. When inducing regenerative processes without a selective factor, the ability to morphogenesis was maintained by 65.7 % of cell lines. The presence of sodium chloride in the culture substrate reduced the proportion of morphogenically active biomaterials to 56.6 %, with inhibition of the morphogenic activity of microcalluses by 31.6 %. On average, genotype in the control variant of induction of morphogenesis from one microcalus formed 1.9 micropagons, and in the presence of sodium chloride — 1.3. The reserached cell lines of camelina sativa differed in the level of salt resistance, retention of the ability to regenerate and morphogenic activity of microcalluses, which influenced the results of obtaining plants-regenerants. In total, 381 regenerants were obtained as a result of studies performed on the cell lines of the camelina sativa, which were characterized by the highest level of salt resistance. The presence of sodium chloride in regenerative media reduced the production of whole plants by an average of 45.4% by genotype.

Keywords

• camelina sativa
• morphogenesis
• callus line
• regenerant plant
• sodium chloride