Dielectrophoretic and Electrical Impedance Differentiation of Cancerous Cells Based on Biophysical Phenotype
Ina Turcan,
Iuliana Caras,
Thomas Gabriel Schreiner,
Catalin Tucureanu,
Aurora Salageanu,
Valentin Vasile,
Marioara Avram,
Bianca Tincu,
Marius Andrei Olariu
Affiliations
Ina Turcan
Department of Electrical Measurements and Materials, Faculty of Electrical Engineering and Information Technology, Gheorghe Asachi Technical University of Iasi, 21-23 Profesor Dimitrie Mangeron Blvd., 700050 Iasi, Romania
Iuliana Caras
“Cantacuzino” National Medical-Military Institute for Research and Development, 103 Splaiul Independentei, 050096 Bucharest, Romania
Thomas Gabriel Schreiner
Department of Electrical Measurements and Materials, Faculty of Electrical Engineering and Information Technology, Gheorghe Asachi Technical University of Iasi, 21-23 Profesor Dimitrie Mangeron Blvd., 700050 Iasi, Romania
Catalin Tucureanu
“Cantacuzino” National Medical-Military Institute for Research and Development, 103 Splaiul Independentei, 050096 Bucharest, Romania
Aurora Salageanu
“Cantacuzino” National Medical-Military Institute for Research and Development, 103 Splaiul Independentei, 050096 Bucharest, Romania
Valentin Vasile
“Cantacuzino” National Medical-Military Institute for Research and Development, 103 Splaiul Independentei, 050096 Bucharest, Romania
Marioara Avram
National Institute for Research and Development in Microtechnologies—IMT Bucharest, 126A Erou Iancu Nicolae Street, 077190 Bucharest, Romania
Bianca Tincu
National Institute for Research and Development in Microtechnologies—IMT Bucharest, 126A Erou Iancu Nicolae Street, 077190 Bucharest, Romania
Marius Andrei Olariu
Department of Electrical Measurements and Materials, Faculty of Electrical Engineering and Information Technology, Gheorghe Asachi Technical University of Iasi, 21-23 Profesor Dimitrie Mangeron Blvd., 700050 Iasi, Romania
Here, we reported a study on the detection and electrical characterization of both cancer cell line and primary tumor cells. Dielectrophoresis (DEP) and electrical impedance spectroscopy (EIS) were jointly employed to enable the rapid and label-free differentiation of various cancer cells from normal ones. The primary tumor cells that were collected from two colorectal cancer patients, cancer cell lines (SW-403, Jurkat, and THP-1), and healthy peripheral blood mononuclear cells (PBMCs) were trapped first at the level of interdigitated microelectrodes with the help of dielectrophoresis. Correlation of the cells dielectric characteristics that was obtained via electrical impedance spectroscopy (EIS) allowed evident differentiation of the various types of cell. The differentiations were assigned to a “dielectric phenotype” based on their crossover frequencies. Finally, Randles equivalent circuit model was employed for highlighting the differences with regard to a series group of charge transport resistance and constant phase element for cancerous and normal cells.
