A synthetic cytokinin primes photosynthetic and growth response in grapevine under ion-independent salinity stress

Abstract

Aiding optimal plant–environment interaction would favor plant resilience against environmental constrains including salt stress. We test the hypothesis that 6-Benzylaminopurine (BAP) primes grapevine’s salt tolerance in vines (Vitis vinifera) received salt water (NaCl 100 mM) through the modulation of gene expression of BAP (AHK4, AHP1) and salt-stress (CAT, APX) inducible genes and morpho-physiological traits. A subgroup of vines had previously (48 h) been primed with BAP (80 mg/L) before salt stress. The gene expressions were 30% (CAT) and 56% (APX) lower in primed salt-stressed vines than that in un-primed. Salt treatment did not increase leaf Na+ but it lowered stomatal conductance (gs), photosynthesis (A), stem water potential (less negative) and photosystem-II efficiency (Fv/Fm). Chlorophyll-a concentrations were 30% higher in BAP-primed compared to un-primed. Adverse effects of salt were significantly reduced, maintaining high A/gs, Fv/Fm and growth. After the relief of the stress, the BAP primed vines had a fast recovery.

Keywords

• APX
• BAP
• CAT
• gene activation
• leaf gas exchanges
• salt tolerance