Nature Communications (Nov 2017)

Thermostable exoshells fold and stabilize recombinant proteins

  • Siddharth Deshpande,
  • Nihar D. Masurkar,
  • Vallerinteavide Mavelli Girish,
  • Malan Desai,
  • Goutam Chakraborty,
  • Juliana M. Chan,
  • Chester L. Drum

DOI
https://doi.org/10.1038/s41467-017-01585-2
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 8

Abstract

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Improving recombinant protein expression and stabilization remains a significant challenge. Here, the authors engineer Archaeoglobus fulgidus ferritin as a thermostable exoshell to provide steric accommodation and charge complementation for recombinant proteins, which can improve yields by 100 fold.