Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution

Abstract

It remains a challenge to obtain high-resolution structures of molecules smaller than 200 kDa using single particle cryo-EM. Here, the authors apply the Cs-corrector-VPP coupled cryo-EM and solve structures of the 52 kDa streptavidin (SA) protein at near-atomic resolution.