Conjugates of Copper Alginate with Arginine-Glycine-Aspartic Acid (RGD) for Potential Use in Regenerative Medicine

Justyna Fraczyk; Joanna Wasko; Malgorzata Walczak; Zbigniew  J. Kaminski; Dorota Puchowicz; Irena Kaminska; Maciej Bogun; Marcin Kolasa; Ewa Stodolak-Zych; Anna Scislowska-Czarnecka; Beata Kolesinska

doi:10.3390/ma13020337

Materials (Jan 2020)

Conjugates of Copper Alginate with Arginine-Glycine-Aspartic Acid (RGD) for Potential Use in Regenerative Medicine

Justyna Fraczyk,
Joanna Wasko,
Malgorzata Walczak,
Zbigniew J. Kaminski,
Dorota Puchowicz,
Irena Kaminska,
Maciej Bogun,
Marcin Kolasa,
Ewa Stodolak-Zych,
Anna Scislowska-Czarnecka,
Beata Kolesinska

Affiliations

Justyna Fraczyk: Institute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90‐924 Lodz, Poland
Joanna Wasko: Institute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90‐924 Lodz, Poland
Malgorzata Walczak: Institute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90‐924 Lodz, Poland
Zbigniew J. Kaminski: Institute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90‐924 Lodz, Poland
Dorota Puchowicz: Lukasiewicz Research Network-Textile Research Institute, Brzezinska 5/15, 92-103 Lodz, Poland
Irena Kaminska: Lukasiewicz Research Network-Textile Research Institute, Brzezinska 5/15, 92-103 Lodz, Poland
Maciej Bogun: Lukasiewicz Research Network-Textile Research Institute, Brzezinska 5/15, 92-103 Lodz, Poland
Marcin Kolasa: Military Institute of Hygiene and Epidemiology Department of Pharmacology and Toxicology, Kozielska 4, 01-163 Warsaw, Poland
Ewa Stodolak-Zych: Department of Biomaterials, AGH‐University of Science and Technology, A. Mickiewicz 30, 30-059 Krakow, Poland
Anna Scislowska-Czarnecka: Academy of Physical Education, Department of Physiotherapy, Section of Anatomy, 31-008 Krakow, Poland
Beata Kolesinska: Institute of Organic Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90‐924 Lodz, Poland

DOI: https://doi.org/10.3390/ma13020337
Journal volume & issue: Vol. 13, no. 2
p. 337

Abstract

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Current restrictions on the use of antibiotics, associated with increases in bacterial resistance, require new solutions, including materials with antibacterial properties. In this study, copper alginate fibers obtained using the classic wet method were used to make nonwovens which were modified with arginine-glycine-aspartic acid (RGD) derivatives. Stable polysaccharide-peptide conjugates formed by coupling with 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium toluene-4-sulfonate (DMT/NMM/TosO−), and materials with physically embedded RGD derivatives, were obtained. The materials were found to be characterized by very high antibacterial activity against S. aureus and K. pneumoniae. Cytotoxicity studies confirmed that the materials are not cytotoxic. Copper alginate conjugates with RGD peptides have strong potential for use in regenerative medicine, due to their biocompatibility and innate antibacterial activity.

Published in Materials

ISSN: 1996-1944 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering; Technology: Engineering (General). Civil engineering (General); Science: Natural history (General): Microscopy; Science: Physics: Descriptive and experimental mechanics
Website: http://www.mdpi.com/journal/materials/

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