Frontiers in Veterinary Science (Nov 2024)
A subunit vaccine based on P97R1, P46, P42, and P65 from Mycoplasma hyopneumoniae can induce significant immune response in piglets
- Yintao He,
- Yintao He,
- Kaiyuan Xie,
- Kaiyuan Xie,
- Zhongmao Yuan,
- Zhongmao Yuan,
- Ting Ouyang,
- Ting Ouyang,
- Anran Dong,
- Anran Dong,
- Bing Ling,
- Bing Ling,
- Weijun Zeng,
- Weijun Zeng,
- Yiqi Fang,
- Yiqi Fang,
- Yiwan Song,
- Yiwan Song,
- Lianxiang Wang,
- Hongxing Ding,
- Hongxing Ding,
- Mingqiu Zhao,
- Mingqiu Zhao,
- Shuangqi Fan,
- Shuangqi Fan,
- Lin Yi,
- Lin Yi,
- Dongfang Zhao,
- Dongfang Zhao,
- Dongfang Zhao,
- Jinding Chen,
- Jinding Chen
Affiliations
- Yintao He
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yintao He
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- Kaiyuan Xie
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Kaiyuan Xie
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- Zhongmao Yuan
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Zhongmao Yuan
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- Ting Ouyang
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Ting Ouyang
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- Anran Dong
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Anran Dong
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- Bing Ling
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Bing Ling
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- Weijun Zeng
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Weijun Zeng
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- Yiqi Fang
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yiqi Fang
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- Yiwan Song
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Yiwan Song
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- Lianxiang Wang
- Wen’s Group Academy, Wen’s Foodstuffs Group Co., Ltd., Xinxing, China
- Hongxing Ding
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Hongxing Ding
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- Mingqiu Zhao
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Mingqiu Zhao
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- Shuangqi Fan
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Shuangqi Fan
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- Lin Yi
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Lin Yi
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- Dongfang Zhao
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Dongfang Zhao
- Wen’s Group Academy, Wen’s Foodstuffs Group Co., Ltd., Xinxing, China
- Dongfang Zhao
- Yunfu Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
- Jinding Chen
- College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
- Jinding Chen
- Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, South China Agricultural University, Guangzhou, China
- DOI
- https://doi.org/10.3389/fvets.2024.1493650
- Journal volume & issue
-
Vol. 11
Abstract
Mycoplasma pneumonia (MPS), caused by Mycoplasma hyopneumoniae (Mhp), is a chronic, airborne respiratory disease that poses a significant threat to the global swine industry. The P97 and P46 proteins are major antigens of Mhp, with the R1 region of P97 possessing full adhesive capability. Studies have shown that the main antigenic regions of Mhp P42 and P65 proteins exhibit strong immunogenicity. In this study, we first linked the genes encoding P97R1 and P46 proteins to form the P97R1P65 gene and subsequently constructed three shuttle plasmids: pFBD-P97R1P46, pFBD-P97R1P46-p65, and pFBD-P65-P42. These proteins were expressed using the Bac to Bac system and formulated into subunit vaccines for mouse immunization. Mouse experiments indicated that the P97R1P46 + P65-P42 protein combination elicited higher levels of specific antibodies, IL-2, IL-4, and CD8+ T cells compared to other subunit vaccine groups, a finding further validated in subsequent mouse challenge protection experiments. Therefore, we utilized the MultiBac expression system to co-express P97R1P46, P65, and P42 proteins in the pFastMultibacDual vector for immunization experiments in piglets. The piglet immunization experiments demonstrated that the Mhp subunit vaccine prepared in this study could induce specific antibodies against Mhp, with the combination of P97R1P46, P65, and P42 proteins inducing the highest level of humoral immunity. This study provides valuable insights for the development of Mhp subunit vaccines.
Keywords
- porcine Mycoplasma pneumonia
- baculovirus expression system
- subunit vaccines
- immunity
- co-expression of multiple promoters
