Early-life polystyrene nanoplastics exposure impairs pathogen avoidance behavior associated with intestine-derived insulin-like neuropeptide (ins-11) and serotonin signaling in Caenorhabditis elegans

Abstract

Nanoplastics (NPs) contamination is an emerging global concern due to the widespread use of plastic products and their potentially negative health impact on ecosystems. Despite their ubiquity, the effects of early-life NPs exposure on host-pathogen interactions remain largely unknown. In this study, we show that early-life exposure to polystyrene NPs (PS-NPs, 100-nm) at predicted environmentally relevant concentrations (10 µg/L) significantly impairs food preference and reduces avoidance of the pathogenic bacterium Bacillus thuringiensis in Caenorhabditis elegans. Exposure to PS-NPs led to a decrease in avoidance from 40.3 % in controls to 30.6 % at 10 µg/L and further to 23.1 % and 17.4 % at 50 and 100 µg/L, respectively. Mechanistic insights reveal that PS-NPs downregulate intestine-derived insulin-like neuropeptide (ins-11) via the transcription factor HLH-30 and the p38 MAPK signaling pathways, both are essential for avoidance behavior. Notably, acute serotonin treatment restored the avoidance behavior, indicating a role of serotonin signaling in this process. Our study indicates that early-life exposure to PS-NPs (100-nm) adversely affects the avoidance behavior of C. elegans, making them more vulnerable to harmful pathogens, thereby affecting their health. These findings highlight significant ecological and health hazards by early-life PS-NPs exposure.

Keywords

• Plastic pollution
• Ecotoxicity
• Host-pathogen interactions
• Caenorhabditis elegans