The <it>Francisella tularensis</it> LVS Δ<it>pdpC</it> mutant exhibits a unique phenotype during intracellular infection

Abstract

Abstract Background A prerequisite for the virulence of the facultative intracellular bacterium Francisella tularensis is effective intramacrophage proliferation, which is preceded by phagosomal escape into the cytosol, and ultimately leads to host cell death. Many components essential for the intracellular life cycle are encoded by a gene cluster, the Francisella pathogenicity island (FPI), constituting a type VI secretion system. Results We characterized the FPI mutant ΔpdpC of the live vaccine strain (LVS) of F. tularensis and found that it exhibited lack of intracellular replication, incomplete phagosomal escape, and marked attenuation in the mouse model, however, unlike a phagosomally contained FPI mutant, it triggered secretion of IL-1β, albeit lower than LVS, and markedly induced LDH release. Conclusions The phenotype of the ΔpdpC mutant appears to be unique compared to previously described F. tularensis FPI mutants.

Keywords

• <it>Francisella tularensis</it>
• Type VI secretion
• Cytopathogenicity
• Intracellular replication
• PdpC