Evaluation in vitro of cytotoxicity of dentin desensitizers on human gingival fibroblasts.

Abstract

The purpose of this study is to compare the cytotoxic effect of three materials, which have been used for treating dental hypersensitivity. Material and method: In vitro study. Clinpro (3M Co, St. Paul, MN. USA), Seal & Protect (Dentsply, DeTrey GmbH. Germany) and UltraEZ (Ultradent Products, Inc., S. South Jordan UT. USA) were used at concentrations of 0.1, 0.05, 0.01 and 0.001g/ml on human gingival fibroblasts. Furthermore, Clinpro and Seal & Protect were applied to this cell culture as polymerized disks. Toxicity was assessed at 24 and 48 hours by the use of the cell viability assay (MTT). Statistical analysis for cell viability was performed using two-way ANOVA and Tukey’s post hoc test. Statistical significance was set at 5%. Results: Seal & Protect and Clinpro were found to be highly toxic at 24 and 48 hours, reaching 70% toxicity at concentrations over 0.01g/ml. Seal & Protect and Clinpro polymerized disks were toxic at 24 and 48 hours. UltraEZ showed an increased between 46% and 67% in cell viability at 24 hours and between 8% and 45% at 48 hours. Statistical analysis showed differences between these three desensitizers when comparing concentration and control group (p<0.05). Discussion: UltraEZ did not have a cytotoxic effect and may be considered a compatible and safe material, whereas polymerized and non-polymerized Clinpro and Seal & Protect should be used with caution.

Keywords

• Dentin Desensitizing Agents
• Cytotoxicity
• Human Gingival Fibroblasts
• Dentin Sensitivity