Тонкие химические технологии (Dec 2007)

Cloning and expression of ricin a- and b-subunits genes in fusion constructions with dihydrofolate reductase gene in escheriсhia coli.

  • M. А. Gracheva,
  • Z. М. Galushkina,
  • N. N. Poletaeva,
  • L. V. Verhovskaya,
  • V. G. Lunin,
  • V. I. Shvets

Journal volume & issue
Vol. 2, no. 6
pp. 40 – 47

Abstract

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Recombinant chimeric constructions RTA-DHFR and RTВ-DHFR consisting of ricin Aand B-subunits genes (RTA and RTB) and dihydrofolate reductase (DHFR) gene were cloned and expressed in Escheriсhia coli. Antigenic properties of RTA and RTB retained in chimeric proteins RTA-DHFR and RTВ-DHFR.