A Simple and Rapid Fungal DNA Isolation Assay Based on ZnO Nanoparticles for the Diagnosis of Invasive Aspergillosis
Zhen Qiao,
Huifang Liu,
Geun Su Noh,
Bonhan Koo,
Qingshuang Zou,
Kyusik Yun,
Yoon Ok Jang,
Sung-Han Kim,
Yong Shin
Affiliations
Zhen Qiao
Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, Korea
Huifang Liu
Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, Korea
Geun Su Noh
Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, Korea
Bonhan Koo
Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, Korea
Qingshuang Zou
Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, Korea
Kyusik Yun
Department of Bionanotechnology, Gachon University, Gyeonggi-do 13120, Korea
Yoon Ok Jang
Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, Korea
Sung-Han Kim
Department of Infectious Disease, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, Korea
Yong Shin
Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul 05505, Korea
Invasive aspergillosis (IA) is an important cause of morbidity and mortality among immunocompromised people. Imaging and specimen tests used in the clinical diagnosis of aspergillosis with weak and indistinct defects leads to delay in the treatment of early aspergillosis patients. The developing molecular techniques provide a new method for the aspergillosis diagnosis. However, the existing methods are complex, time-consuming and may even be potentially hazardous. In this study, we developed a simple and rapid Aspergillus fumigatus spores DNA isolation assay using synthesized zinc oxide (ZnO). ZnO nanoparticles were used to take the place of the traditional commercial lysis buffer. The quality and quantity of the extracted DNA were sufficient for further diagnostics with polymerase chain reaction (PCR) analysis. This method offers easy, green, and economic alternative DNA isolation for the diagnosis of invasive aspergillosis.
