Nature Communications (Aug 2021)

Expanding the binding specificity for RNA recognition by a PUF domain

  • Wei Zhou,
  • Daniel Melamed,
  • Gabor Banyai,
  • Cindy Meyer,
  • Thomas Tuschl,
  • Marvin Wickens,
  • Junyue Cao,
  • Stanley Fields

DOI
https://doi.org/10.1038/s41467-021-25433-6
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 15

Abstract

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The PUF RNAbinding domain has an eight-repeat structure with the ability to bind an eight-base RNA sequence. Here the authors generate a comprehensive library of variants of a PUF domain to find an RNA binding code and design PUF domains that recognize an arbitrary RNA sequence.