Frontiers in Pharmacology (Aug 2022)
Taohong siwu decoction attenuates AIM2 and NLRC4 inflammasomes by ameliorates deoxyribonucleic acid damage after ischemic stroke
- Ni Wang,
- Ni Wang,
- Ni Wang,
- Ni Wang,
- Ni Wang,
- Furui Chu,
- Furui Chu,
- Furui Chu,
- Furui Chu,
- Furui Chu,
- Lijuan Zhang,
- Lijuan Zhang,
- Changyi Fei,
- Changyi Fei,
- Chao Yu,
- Chao Yu,
- Sujun Xue,
- Sujun Xue,
- Yongzhong Wang,
- Ling Fang,
- Daiyin Peng,
- Daiyin Peng,
- Daiyin Peng,
- Xianchun Duan,
- Xianchun Duan,
- Xianchun Duan,
- Xianchun Duan,
- Xianchun Duan,
- Weidong Chen,
- Weidong Chen,
- Weidong Chen
Affiliations
- Ni Wang
- Department of Pharmacy, The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, China
- Ni Wang
- School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China
- Ni Wang
- MOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials, Hefei, China
- Ni Wang
- Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, China
- Ni Wang
- Key Laboratory of Xin’an Medicine (Anhui University of Chinese Medicine), Ministry of Education, Hefei, China
- Furui Chu
- Department of Pharmacy, The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, China
- Furui Chu
- School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China
- Furui Chu
- MOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials, Hefei, China
- Furui Chu
- Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, China
- Furui Chu
- Key Laboratory of Xin’an Medicine (Anhui University of Chinese Medicine), Ministry of Education, Hefei, China
- Lijuan Zhang
- Department of Pharmacy, The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, China
- Lijuan Zhang
- School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China
- Changyi Fei
- Department of Pharmacy, The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, China
- Changyi Fei
- School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China
- Chao Yu
- Department of Pharmacy, The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, China
- Chao Yu
- School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China
- Sujun Xue
- Department of Pharmacy, The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, China
- Sujun Xue
- School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China
- Yongzhong Wang
- Department of Pharmacy, The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, China
- Ling Fang
- Department of Pharmacy, The First Affiliated Hospital of Anhui Medical University, Hefei, China
- Daiyin Peng
- School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China
- Daiyin Peng
- MOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials, Hefei, China
- Daiyin Peng
- Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, China
- Xianchun Duan
- Department of Pharmacy, The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, China
- Xianchun Duan
- School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China
- Xianchun Duan
- MOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials, Hefei, China
- Xianchun Duan
- Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, China
- Xianchun Duan
- Key Laboratory of Xin’an Medicine (Anhui University of Chinese Medicine), Ministry of Education, Hefei, China
- Weidong Chen
- School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China
- Weidong Chen
- MOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials, Hefei, China
- Weidong Chen
- Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, China
- DOI
- https://doi.org/10.3389/fphar.2022.954867
- Journal volume & issue
-
Vol. 13
Abstract
Taohong siwu decoction (THSWD) has been shown to have a therapeutic effect on ischemic strokes (IS). However, it is not clear to us whether THSWD reduces deoxyribonucleic acid (DNA) damage after stroke and reduces the inflammatory response caused by the damage. Therefore, we constructed an IS model (I/R) in rats and performed oxygen-glucose deprivation/reoxygenation (OGD/R) on BV2 cells. Then ELISA, immunofluorescence staining, immunohistochemistry staining, and RT-qPCR were performed to detect the expressions of absent in melanoma 2 (AIM2), NLRC4, and Caspase-1 inflammasomes and other inflammatory factors. Experimental stroke causes DNA damage, and we found that the aforementioned inflammasomes as well as inflammatory factors were significantly inhibited after treatment with THSWD by comparing the model group with the model administration group. In addition, we examined the expression of AIM2, NLRC4, and Caspase-1 in BV2 cells of OGD/R and found that the expression of the aforementioned inflammasomes was significantly decreased in OGD/R by administration of THSWD-containing serum. Our data suggest that THSWD can reduced DNA damage after stroke as well as the inflammatory response caused by the damage.
Keywords
