Amino acid modified OCMC-g-Suc-β-CD nanohydrogels carrying lapatinib and ginsenoside Rg1 exhibit high anticancer activity in a zebrafish model

Li Cui; Li Cui; Xiaolan Liu; Rongjun Yan; Qixu Chen; Lizhen Wang; Shah Nawaz; Dawei Qin; Daijie Wang; Daijie Wang

doi:10.3389/fphar.2023.1149191

Frontiers in Pharmacology (May 2023)

Amino acid modified OCMC-g-Suc-β-CD nanohydrogels carrying lapatinib and ginsenoside Rg1 exhibit high anticancer activity in a zebrafish model

Li Cui,
Li Cui,
Xiaolan Liu,
Rongjun Yan,
Qixu Chen,
Lizhen Wang,
Shah Nawaz,
Dawei Qin,
Daijie Wang,
Daijie Wang

Affiliations

Li Cui: School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China
Li Cui: Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China
Xiaolan Liu: Jinan Authority Hospital, Jinan, China
Rongjun Yan: Jinan International Travel Healthcare Center, Jinan, China
Qixu Chen: Biological Engineering Technology Innovation Center of Shandong Province, Heze Branch of Qilu University of Technology (Shandong Academy of Sciences), Heze, China
Lizhen Wang: Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China
Shah Nawaz: Department of Chemistry, Karakoram International University, Gilgit, Pakistan
Dawei Qin: School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China
Daijie Wang: Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China
Daijie Wang: Biological Engineering Technology Innovation Center of Shandong Province, Heze Branch of Qilu University of Technology (Shandong Academy of Sciences), Heze, China

DOI: https://doi.org/10.3389/fphar.2023.1149191
Journal volume & issue: Vol. 14

Abstract

Read online

Nanohydrogels show great potential as efficient drug carriers due to their biocompatibility, low toxicity, and high water absorbability. In this paper, we prepared two O-carboxymethylated chitosan (OCMC)-based polymers functionalized with β-cyclodextrin (β-CD) and amino acid. The structures of the polymers were characterized by Fourier Transform Infrared (FTIR) Spectroscopy. Morphological study was carried out on a Transmission Electron Microscope (TEM), and the results indicated that the two polymers had irregular spheroidal structure with some pores distributed on their surface. The average particle diameter was below 500 nm, and the zeta potential was above +30 mV. The two polymers were further used for preparing nanohydrogels loaded with anticancer drugs lapatinib and ginsenoside Rg1, and the resulting nanohydrogels showed high drug loading efficiency and pH-sensitive (pH = 4.5) drug release behavior. In vitro cytotoxicity investigation revealed that the nanohydrogels exhibited high cytotoxicity against lung cancer (A549) cells. In vivo anticancer investigation was performed in a transgenic Tg(fabp10:rtTA2s-M2; TRE2:EGFP-krasV12) zebrafish model. The results showed that the synthesized nanohydrogels significantly inhibited the expression of EGFP-krasv12 oncogene in zebrafish liver, and the L-arginine modified OCMC-g-Suc-β-CD nanohydrogels loading lapatinib and ginsenoside Rg1 showed the best results.

Published in Frontiers in Pharmacology

ISSN: 1663-9812 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://journal.frontiersin.org/journals/pharmacology

About the journal

Abstract

Keywords