A protocol to study bacteriophage adaptation to new hosts

Abstract

Summary: A general protocol for the experimental assessment of bacteriophage adaptation to new hosts is described. We use as a model system the lytic phage T7 and an engineered E. coli strain modified to hamper the recruitment of a known proviral factor. Our protocol includes steps of phage amplification, plaque and liquid lysis assays, and DNA extraction for next-generation sequencing of the viral genome over several rounds of laboratory evolution thus allowing the investigation of the sequence determinants of viral adaptation.For complete information on the generation and use of this protocol, please refer to Luzon-Hidalgo et al. (2021).

Keywords

• Biophysics
• Microbiology
• Model organisms
• Molecular biology
• Biotechnology and bioengineering
• Evolutionary biology