Bulblet regeneration from ex vitro root explant in lily hybrids

Abstract

The influence of growth regulators on in vitro bulblet formation from ex vitro roots was studied in asiatic and oriental hybrids of Lilium The root segments (3-4 mm) isolated from the middle zone of 2-3 cm ex vitro root were cultured on Murashige and Skoog (MS) medium containing 1 or 1.5 mg/dm3 naphthalene acetic acid (NAA) and/or benzyladenine (BA). Bulblets were not produced in the presence of NAA and BA alone. A significant increase in the per cent explants producing bulblets was observed with 1.5 mg/dm3 NAA and 1 mg/dm3 BA. Maximum number of bulblets and average fresh weight per bulblet was observed with 2 mg/dm3 NAA and 1.5 mg/dm3 BA after 90 days of culture. No differences were found among cultivars in bulblet regeneration of explant or bulblet number although more weighty bulblets occurred in cv. Apeldoorn. About 82% bulblet survival was recorded in coco peat after 30 days of transfer to pots.

Keywords

• lily hybrids
• cvs. alaska
• apeldoorn
• beartix
• siberia
• marco polo
• naa
• ba
• iba
• in vitro
• bulblet regeneration
• ex vitro root
• rooting
• hardening