Sensitive and prolonged intracellular electrophysiological recording by three‐dimensional nanodensity regulation
Xingxing Liu,
Dongxin Xu,
Jiaru Fang,
Yuheng Liao,
Mingyue Zhang,
Hongbo Li,
Wenjian Yang,
Yue Wu,
Zhongyuan Xu,
Ning Hu,
Diming Zhang
Affiliations
Xingxing Liu
Guangdong Provincial Key Laboratory of Industrial Surfactant Institute of Chemical Engineering Guangdong Academy of Sciences GuangzhouChina
Dongxin Xu
State Key Laboratory of Optoelectronic Materials and Technologies Guangdong Province Key Laboratory of Display Material and Technology School of Electronics and Information Technology Sun Yat‐sen University GuangzhouChina
Jiaru Fang
State Key Laboratory of Optoelectronic Materials and Technologies Guangdong Province Key Laboratory of Display Material and Technology School of Electronics and Information Technology Sun Yat‐sen University GuangzhouChina
Yuheng Liao
Research Center for Intelligent Sensing Systems Zhejiang Laboratory HangzhouChina
Mingyue Zhang
State Key Laboratory of Optoelectronic Materials and Technologies Guangdong Province Key Laboratory of Display Material and Technology School of Electronics and Information Technology Sun Yat‐sen University GuangzhouChina
Hongbo Li
State Key Laboratory of Optoelectronic Materials and Technologies Guangdong Province Key Laboratory of Display Material and Technology School of Electronics and Information Technology Sun Yat‐sen University GuangzhouChina
Wenjian Yang
Research Center for Intelligent Sensing Systems Zhejiang Laboratory HangzhouChina
Yue Wu
Research Center for Intelligent Sensing Systems Zhejiang Laboratory HangzhouChina
Zhongyuan Xu
Research Center for Intelligent Sensing Systems Zhejiang Laboratory HangzhouChina
Ning Hu
Department of Chemistry Zhejiang University HangzhouChina
Diming Zhang
Research Center for Intelligent Sensing Systems Zhejiang Laboratory HangzhouChina
Abstract With the advancement of micro/nanotechnologies, multielectrodes arrays (MEAs) with different three‐dimensional (3D) micro/nanostructures have been developed to achieve intracellular action potential recording of cardiomyocytes. However, the effect of the 3D micro/nanostructures density on the intracellular recording has not been fully investigated. In this work, 3D tunable nanodensity electrode arrays (TNDEA) are fabricated by hydrothermal synthesis and standard microfabrication to explore the effect of nanodensity regulation on intracellular biosensing. By low‐voltage electroporation, the signal quality of intracellular potentials recorded by the low‐nanodensity TNDEA showed was significantly improved compared to those recorded by the high‐nanodensity TNDEA. The low‐nanodensity TNDEA improved the amplitude (up to 7.7 mV), signal‐to‐noise ratio (SNR) (up to 69.46 dB), recording duration (up to 83 min), and recording yield (∼100%). The 3D nanodensity regulating strategy has achieved sensitive and prolonged intracellular biosensing of action potentials and is expected to be a powerful electrophysiological research tool in the biomedical field.
