Nature Communications (Oct 2021)
Regulation of local GTP availability controls RAC1 activity and cell invasion
- Anna Bianchi-Smiraglia,
- David W. Wolff,
- Daniel J. Marston,
- Zhiyong Deng,
- Zhannan Han,
- Sudha Moparthy,
- Rebecca M. Wombacher,
- Ashley L. Mussell,
- Shichen Shen,
- Jialin Chen,
- Dong-Hyun Yun,
- Anderson O’Brien Cox,
- Cristina M. Furdui,
- Edward Hurley,
- Maria Laura Feltri,
- Jun Qu,
- Thomas Hollis,
- Jules Berlin Nde Kengne,
- Bernard Fongang,
- Rui J. Sousa,
- Mikhail E. Kandel,
- Eugene S. Kandel,
- Klaus M. Hahn,
- Mikhail A. Nikiforov
Affiliations
- Anna Bianchi-Smiraglia
- Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center
- David W. Wolff
- Department of Cancer Biology, Wake Forest University Baptist Medical Center
- Daniel J. Marston
- Department of Pharmacology and Lineberger Cancer Center, University of North Carolina at Chapel Hill
- Zhiyong Deng
- Department of Cancer Biology, Wake Forest University Baptist Medical Center
- Zhannan Han
- Department of Cancer Biology, Wake Forest University Baptist Medical Center
- Sudha Moparthy
- Department of Cancer Biology, Wake Forest University Baptist Medical Center
- Rebecca M. Wombacher
- Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center
- Ashley L. Mussell
- Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center
- Shichen Shen
- New York State Center of Excellence Bioinformatics and Life Sciences, State University of New York at Buffalo
- Jialin Chen
- Department of Cancer Biology, Wake Forest University Baptist Medical Center
- Dong-Hyun Yun
- Department of Cancer Biology, Wake Forest University Baptist Medical Center
- Anderson O’Brien Cox
- Department of Cancer Biology, Wake Forest University Baptist Medical Center
- Cristina M. Furdui
- Department of Cancer Biology, Wake Forest University Baptist Medical Center
- Edward Hurley
- Department of Biochemistry and Neurology, Hunter James Kelly Research Institute, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo
- Maria Laura Feltri
- Department of Biochemistry and Neurology, Hunter James Kelly Research Institute, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo
- Jun Qu
- New York State Center of Excellence Bioinformatics and Life Sciences, State University of New York at Buffalo
- Thomas Hollis
- Department of Biochemistry, Center for Structural Biology, Wake Forest School of Medicine
- Jules Berlin Nde Kengne
- Department of Physics, University of Houston
- Bernard Fongang
- Glenn Biggs Institute for Alzheimer’s and Neurodegenerative Diseases, University of Texas Health Science Center at San Antonio
- Rui J. Sousa
- Department of Biochemistry and Structural Biology, University of Texas Health Science Center at San Antonio
- Mikhail E. Kandel
- Groq, 400 Castro St #600
- Eugene S. Kandel
- Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center
- Klaus M. Hahn
- Department of Pharmacology and Lineberger Cancer Center, University of North Carolina at Chapel Hill
- Mikhail A. Nikiforov
- Department of Cancer Biology, Wake Forest University Baptist Medical Center
- DOI
- https://doi.org/10.1038/s41467-021-26324-6
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 15
Abstract
Changes in intracellular GTP levels are not considered as a regulatory event in RAC1 activation in live cells since total GTP levels are substantially higher than the RAC1 GTP dissociation constant determined in vitro. Here, the authors demonstrate that the availability of free GTP in live cells controls the activity of RAC1 and cell invasion.
