In Vivo and In Vitro Study of a Polylactide-Fiber-Reinforced β-Tricalcium Phosphate Composite Cage in an Ovine Anterior Cervical Intercorporal Fusion Model

Janek Frantzén; Aliisa Pälli; Esa Kotilainen; Harri Heino; Bettina Mannerström; Heini Huhtala; Hannu Kuokkanen; George K. Sándor; Kari Leino; Matias Röyttä; Riitta Parkkola; Riitta Suuronen; Susanna Miettinen; Hannu T. Aro; Suvi Haimi

doi:10.1155/2011/109638

International Journal of Biomaterials (Jan 2011)

In Vivo and In Vitro Study of a Polylactide-Fiber-Reinforced β-Tricalcium Phosphate Composite Cage in an Ovine Anterior Cervical Intercorporal Fusion Model

Janek Frantzén,
Aliisa Pälli,
Esa Kotilainen,
Harri Heino,
Bettina Mannerström,
Heini Huhtala,
Hannu Kuokkanen,
George K. Sándor,
Kari Leino,
Matias Röyttä,
Riitta Parkkola,
Riitta Suuronen,
Susanna Miettinen,
Hannu T. Aro,
Suvi Haimi

Affiliations

Janek Frantzén: Neurosurgical Unit, Department of Surgery, Turku University Central Hospital, P.O. Box 52, 20521 Turku, Finland
Aliisa Pälli: Institute of Biomedical Technology, University of Tampere, 33014 Tampere, Finland
Esa Kotilainen: Neurosurgical Unit, Department of Surgery, Turku University Central Hospital, P.O. Box 52, 20521 Turku, Finland
Harri Heino: Bioretec Ltd., Hermiankatu 22, 33720 Tampere, Finland
Bettina Mannerström: Institute of Biomedical Technology, University of Tampere, 33014 Tampere, Finland
Heini Huhtala: Tampere School of Public Health, University of Tampere, 33014 Tampere, Finland
Hannu Kuokkanen: Department of Plastic Surgery, Tampere University Hospital, P.O. Box 2000, 33521 Tampere, Finland
George K. Sándor: Institute of Biomedical Technology, University of Tampere, 33014 Tampere, Finland
Kari Leino: Department of Anaesthesiology, Intensive Care, Emergency Care and Pain Medicine, Turku University Central Hospital, P.O. Box 52, 20521 Turku, Finland
Matias Röyttä: Department of Pathology, Turku University Central Hospital, P.O. Box 52, 20521 Turku, Finland
Riitta Parkkola: Department of Radiology, Turku University Central Hospital, P.O. Box 52, 20521 Turku, Finland
Riitta Suuronen: Institute of Biomedical Technology, University of Tampere, 33014 Tampere, Finland
Susanna Miettinen: Institute of Biomedical Technology, University of Tampere, 33014 Tampere, Finland
Hannu T. Aro: Orthopedic Research Unit, Department of Orthopedic Surgery and Traumatology, University of Turku, Lemminkäisenkatu 2, 20520 Turku, Finland
Suvi Haimi: Institute of Biomedical Technology, University of Tampere, 33014 Tampere, Finland

DOI: https://doi.org/10.1155/2011/109638
Journal volume & issue: Vol. 2011

Abstract

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A poly-70L/30DL-lactide (PLA70)–β-tricalcium phosphate (β-TCP) composite implant reinforced by continuous PLA-96L/4D-lactide (PLA96) fibers was designed for in vivo spinal fusion. The pilot study was performed with four sheep, using titanium cage implants as controls. The composite implants failed to direct bone growth as desired, whereas the bone contact and the proper integration were evident with controls 6 months after implantation. Therefore, the PLA70/β-TCP composite matrix material was further analyzed in the in vitro experiment by human and ovine adipose stem cells (hASCs and oASCs). The composites proved to be biocompatible as confirmed by live/dead assay. The proliferation rate of oASCs was higher than that of hASCs at all times during the 28 d culture period. Furthermore, the composites had only a minor osteogenic effect on oASCs, whereas the hASC osteogenesis on PLA70/β-TCP composites was evident. In conclusion, the composite implant material can be applied with hASCs for tissue engineering but not be evaluated in vivo with sheep.

Published in International Journal of Biomaterials

ISSN: 1687-8787 (Print); 1687-8795 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Technology: Chemical technology: Biotechnology
Website: https://onlinelibrary.wiley.com/journal/6418

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