OncoTargets and Therapy (Sep 2019)
MiR-19a Promotes Migration And Invasion By Targeting RHOB In Osteosarcoma
Abstract
Ruidong Liu,1 Liefeng Shen,2 Niyan Qu,3 Xia Zhao,4 Jialiang Wang,5 Jun Geng6 1Department of Clinical Laboratory, Jinan City People’s Hospital, Jinan 271100, People’s Republic of China; 2Department of Spinal Surgery, The People’s Hospital of Zhangqiu Area, Jinan 250200, People’s Republic of China; 3Department of PICU, Qingdao Women and Children’s Hospital, Qingdao 266012, People’s Republic of China; 4Medical Insurance Department, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, People’s Republic of China; 5Department of Radiology, The People’s Hospital of Zhangqiu Area, Jinan 250200, People’s Republic of China; 6Medical Laboratory Diagnosis Center, Jinan Central Hospital, Jinan 250013, People’s Republic of ChinaCorrespondence: Jun GengMedical Laboratory Diagnosis Center, Jinan Central Hospital, 105 Jiefang Road, Lixia District, Jinan 250013, Jinan, People’s Republic of ChinaTel +86 531 8569 5114Email [email protected]: Osteosarcoma is the most common bone tumor with high metastasis and recurrence rate. MicroRNA-19a (miR-19a) has been reported to act as tumor oncogene in multiple cancers. The objective of the study was to explore the molecular mechanisms of miR-19a in osteosarcoma cell migration and invasion.Materials and methods: Real-time quantitative polymerase chain reaction (RT-qPCR) and Western blotting were employed to measure the levels of miR-19a and RhoB in osteosarcoma tissues and cell lines. Transwell assay was employed to analyze the tissues and cell lines’ migratory and invasive abilities. Dual luciferase reporter assay was utilized to analyze the association between miR-19a and RhoB.Results: MiR-19a was overexpressed in osteosarcoma tissues and cell lines. MiR-19a promoted osteosarcoma cell migration and invasion in vitro. RhoB was thus confirmed as a direct and functional target of miR-19a, and it could partially reverse the function of miR-19a. Knockdown miR-19a inhibited osteosarcoma cell epithelial-mesenchymal transition (EMT) and suppressed osteosarcoma xenograft growth.Conclusion: MiR-19a enhanced cell migration, invasion and EMT through RhoB in osteosarcoma. The newly identified miR-19a/RhoB axis provides novel insight into the progression of osteosarcoma and offers a promising target for osteosarcoma therapy.Keywords: miR-19a, RhoB, EMT, osteosarcoma, migration, invasion
