Antioxidant Effect of the Ethyl Acetate Extract of <i>Potentilla indica</i> on Kidney Mitochondria of Streptozotocin-Induced Diabetic Rats
Cinthia I. Landa-Moreno,
Cristian M. Trejo-Hurtado,
Jenaro Lemus-de la Cruz,
Donovan J. Peña-Montes,
Marina Murillo-Villicaña,
Maribel Huerta-Cervantes,
Rocío Montoya-Pérez,
Rafael Salgado-Garciglia,
Salvador Manzo-Avalos,
Christian Cortés-Rojo,
Juan Luis Monribot-Villanueva,
José Antonio Guerrero-Analco,
Alfredo Saavedra-Molina
Affiliations
Cinthia I. Landa-Moreno
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Morelia 58030, Michoacán, Mexico
Cristian M. Trejo-Hurtado
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Morelia 58030, Michoacán, Mexico
Jenaro Lemus-de la Cruz
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Morelia 58030, Michoacán, Mexico
Donovan J. Peña-Montes
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Morelia 58030, Michoacán, Mexico
Marina Murillo-Villicaña
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Morelia 58030, Michoacán, Mexico
Maribel Huerta-Cervantes
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Morelia 58030, Michoacán, Mexico
Rocío Montoya-Pérez
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Morelia 58030, Michoacán, Mexico
Rafael Salgado-Garciglia
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Morelia 58030, Michoacán, Mexico
Salvador Manzo-Avalos
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Morelia 58030, Michoacán, Mexico
Christian Cortés-Rojo
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Morelia 58030, Michoacán, Mexico
Juan Luis Monribot-Villanueva
Red de Estudios Moleculares Avanzados, Clúster BioMimic, Instituto de Ecología, A.C., Xalapa 91073, Veracruz, Mexico
José Antonio Guerrero-Analco
Red de Estudios Moleculares Avanzados, Clúster BioMimic, Instituto de Ecología, A.C., Xalapa 91073, Veracruz, Mexico
Alfredo Saavedra-Molina
Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Francisco J. Múgica S/N, Morelia 58030, Michoacán, Mexico
Diabetes mellitus (DM) is a metabolic disorder characterized by persistent hyperglycemia. This state may lead to an increase in oxidative stress, which contributes to the development of diabetes complications, including diabetic kidney disease. Potentilla indica is a traditional medicinal herb in Asia, employed in the treatment of several diseases, including DM. In this study, we investigated the antioxidant effect of the ethyl acetate extract of Potentilla indica both in vitro and on kidneys of streptozotocin-induced diabetic male rats. Firstly, phytochemicals were identified via UPLC-MS/MS, and their in vitro antioxidant capabilities were evaluated. Subsequently, male Wistar rats were assigned into four groups: normoglycemic control, diabetic control, normoglycemic treated with the extract, and diabetic treated with the extract. At the end of the treatment, fasting blood glucose (FBG) levels, creatinine, blood urea nitrogen (BUN), and uric acid were estimated. Furthermore, the kidneys were removed and utilized for the determination of mitochondrial reactive oxygen species (ROS) production, mitochondrial respiratory chain complex activities, mitochondrial lipid peroxidation, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities. The in vitro findings showed that the major phytochemicals present in the extract were phenolic compounds, which exhibited a potent antioxidant activity. Moreover, the administration of the P. indica extract reduced creatinine and BUN levels, ROS production, and lipid peroxidation and improved mitochondrial respiratory chain complex activity and GSH-Px, SODk, and CAT activities when compared to the diabetic control group. In conclusion, our data suggest that the ethyl acetate extract of Potentilla indica possesses renoprotective effects by reducing oxidative stress on the kidneys of streptozotocin-induced diabetic male rats.
