Nature Communications (May 2018)
Bacterial encapsulins as orthogonal compartments for mammalian cell engineering
- Felix Sigmund,
- Christoph Massner,
- Philipp Erdmann,
- Anja Stelzl,
- Hannes Rolbieski,
- Mitul Desai,
- Sarah Bricault,
- Tobias P. Wörner,
- Joost Snijder,
- Arie Geerlof,
- Helmut Fuchs,
- Martin Hrabĕ de Angelis,
- Albert J. R. Heck,
- Alan Jasanoff,
- Vasilis Ntziachristos,
- Jürgen Plitzko,
- Gil G. Westmeyer
Affiliations
- Felix Sigmund
- Institute of Biological and Medical Imaging, Helmholtz Zentrum München
- Christoph Massner
- Institute of Biological and Medical Imaging, Helmholtz Zentrum München
- Philipp Erdmann
- Department of Structural Biology, Max Planck Institute of Biochemistry
- Anja Stelzl
- Institute of Biological and Medical Imaging, Helmholtz Zentrum München
- Hannes Rolbieski
- Institute of Biological and Medical Imaging, Helmholtz Zentrum München
- Mitul Desai
- Department of Biological Engineering, Massachusetts Institute of Technology
- Sarah Bricault
- Department of Biological Engineering, Massachusetts Institute of Technology
- Tobias P. Wörner
- Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University
- Joost Snijder
- Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University
- Arie Geerlof
- Institute of Structural Biology, Helmholtz Zentrum München
- Helmut Fuchs
- Institute of Experimental Genetics, Helmholtz Zentrum München
- Martin Hrabĕ de Angelis
- Institute of Experimental Genetics, Helmholtz Zentrum München
- Albert J. R. Heck
- Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University
- Alan Jasanoff
- Department of Biological Engineering, Massachusetts Institute of Technology
- Vasilis Ntziachristos
- Institute of Biological and Medical Imaging, Helmholtz Zentrum München
- Jürgen Plitzko
- Department of Structural Biology, Max Planck Institute of Biochemistry
- Gil G. Westmeyer
- Institute of Biological and Medical Imaging, Helmholtz Zentrum München
- DOI
- https://doi.org/10.1038/s41467-018-04227-3
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 14
Abstract
Artificial compartments have been expressed in prokaryotes and yeast, but similar capabilities have been missing for mammalian cell engineering. Here the authors use bacterial encapsulins to engineer genetically controlled multifunctional orthogonal compartments in mammalian cells.
