EFFECT OF LACTOBACILLUS PLANTARUM ON THE AGENT OF DAMPING-OFF OF PINE SEEDLINGS

Abstract

Aim. To study the effect of L. plantarum on micromycetes of Fusarium sp. strain isolated from Scots pine seedlings infected with damping-off disease. Methods. The antagonistic effect of lactobacilli was tested by well agar diffusion method. Effect of L. plantarum on germination of infected seeds was studied by presowing treatment of soil with overnight culture of lactobacilli and washed cells of lactobacilli. Overnight culture was also used for treatment of pine seedlings. Results. Overnight cultures of L. plantarum ONU 12, ONU 311, ONU 355 and their consortia caused the inhibition zones on a lawn of Fusarium sp. 17 isolated from damping-off seedling. Pre-sowing treatment of soil with overnight L. plantarum ONU 311 culture increased germination of seeds in 17,3%, surviving of plants – in 7% and mean height of seedlings – in 8%. Inoculation of soil with washed cells and treatment of seedlings were non-effective. Conclusion. Bacteria of L. plantarum species could be studied as perspective microorganisms for improving germination of Scots pine seeds and protection of seedlings under the certain condition of soil treatment.

Keywords

• fusarium
• lactobacillus plantarum
• pinus sylvestris l.
• полягання
• антагонізм