The aim of this study was to investigate the possible impact of silver nanoparticles (Ag-NPs) on reproduction and development of different model organisms, such as sea urchins, barnacles, brine shrimps and zebrafish. In vivo toxicological tests were performed; the biological models were exposed to serial dilutions of commercial Ag-NPs. Our findings revealed that: i) all tests were suitable to verify the impact of Ag-NPs on models in term of exposure; ii) all tests can provide an idea of the role of NPs on environmental health; iii) an integrated interdisciplinary approach, using several model organisms, is recommended in order to have an overview of the effects of NPs on water environment.
