Nature Communications (Sep 2019)
Linkage between endosomal escape of LNP-mRNA and loading into EVs for transport to other cells
- Marco Maugeri,
- Muhammad Nawaz,
- Alexandros Papadimitriou,
- Annelie Angerfors,
- Alessandro Camponeschi,
- Manli Na,
- Mikko Hölttä,
- Pia Skantze,
- Svante Johansson,
- Martina Sundqvist,
- Johnny Lindquist,
- Tomas Kjellman,
- Inga-Lill Mårtensson,
- Tao Jin,
- Per Sunnerhagen,
- Sofia Östman,
- Lennart Lindfors,
- Hadi Valadi
Affiliations
- Marco Maugeri
- Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg
- Muhammad Nawaz
- Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg
- Alexandros Papadimitriou
- Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg
- Annelie Angerfors
- Advanced Drug Delivery, Pharmaceutical Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg
- Alessandro Camponeschi
- Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg
- Manli Na
- Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg
- Mikko Hölttä
- Translational Biomarkers and Bioanalysis, Clinical Pharmacology & Safety Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg
- Pia Skantze
- Advanced Drug Delivery, Pharmaceutical Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg
- Svante Johansson
- Advanced Drug Delivery, Pharmaceutical Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg
- Martina Sundqvist
- Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg
- Johnny Lindquist
- Translational Biomarkers and Bioanalysis, Clinical Pharmacology & Safety Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg
- Tomas Kjellman
- Advanced Drug Delivery, Pharmaceutical Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg
- Inga-Lill Mårtensson
- Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg
- Tao Jin
- Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg
- Per Sunnerhagen
- Department of Chemistry and Molecular Biology, University of Gothenburg
- Sofia Östman
- Animal Sciences and Technologies, Clinical Pharmacology & Safety Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg
- Lennart Lindfors
- Advanced Drug Delivery, Pharmaceutical Sciences, BioPharmaceuticals R&D, AstraZeneca, Gothenburg
- Hadi Valadi
- Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg
- DOI
- https://doi.org/10.1038/s41467-019-12275-6
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 15
Abstract
Lipid nanoparticles (LNPs) are potential platforms for RNA-based therapeutics, but the fate of LNP-RNAs upon internalization into the cell is unclear. Here, the authors show that LNP-mRNAs and ionizable lipids escape the endosomes and are re-released via extracellular vesicles which could deliver the functional mRNA to other cells.
