Ubiquitin-mediated degradation of SlPsbS regulates low night temperature tolerance in tomatoes
Jiazhi Lu,
Junchi Yu,
Pengkun Liu,
Jiamao Gu,
Yu Chen,
Tianyi Zhang,
Jialong Li,
Taotao Wang,
Wenqiang Yang,
Rongcheng Lin,
Feng Wang,
Mingfang Qi,
Tianlai Li,
Yufeng Liu
Affiliations
Jiazhi Lu
The Modern Facilities Horticultural Engineering Technology Center, Shenyang Agricultural University, Shenyang 110866, China; The Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang 110866, China; Yazhouwan National Laboratory, Sanya 572024, China
Junchi Yu
Key Laboratory of Agriculture Biotechnology, College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China
Pengkun Liu
The Modern Facilities Horticultural Engineering Technology Center, Shenyang Agricultural University, Shenyang 110866, China; The Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang 110866, China
Jiamao Gu
The Modern Facilities Horticultural Engineering Technology Center, Shenyang Agricultural University, Shenyang 110866, China; The Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang 110866, China
Yu Chen
The Modern Facilities Horticultural Engineering Technology Center, Shenyang Agricultural University, Shenyang 110866, China; The Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang 110866, China
Tianyi Zhang
The Modern Facilities Horticultural Engineering Technology Center, Shenyang Agricultural University, Shenyang 110866, China; The Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang 110866, China
Jialong Li
Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
Taotao Wang
Key Laboratory of Horticulture Plant Biology, Ministry of Education, Huazhong Agriculture University, Wuhan 430070, China
Wenqiang Yang
Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
Rongcheng Lin
Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
Feng Wang
The Modern Facilities Horticultural Engineering Technology Center, Shenyang Agricultural University, Shenyang 110866, China; The Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang 110866, China
Mingfang Qi
The Modern Facilities Horticultural Engineering Technology Center, Shenyang Agricultural University, Shenyang 110866, China; The Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang 110866, China
Tianlai Li
The Modern Facilities Horticultural Engineering Technology Center, Shenyang Agricultural University, Shenyang 110866, China; The Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang 110866, China; Corresponding author
Yufeng Liu
The Modern Facilities Horticultural Engineering Technology Center, Shenyang Agricultural University, Shenyang 110866, China; The Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang 110866, China; Corresponding author
Summary: PsbS protein is essential for the rapid induction of non-photochemical quenching (NPQ) under low night temperatures (LNTs), but its stability is often affected by adverse environmental conditions. However, the regulatory mechanism for the stability of PsbS or chloroplast proteins remains to be fully characterized. We show that LNT decreases NPQ levels and SlPsbS protein abundance in tomato leaves. LNT-activated chloroplast vesicles (SlCVs) targeting the chloroplasts induce the formation of CV-containing vesicles (CCVs) containing SlPsbS, exported from the chloroplasts. Subsequently, SlCV and SlPsbS contact COP9 signalosome subunit 5A (SlCSN5A) in the cytosol and are ubiquitinated and degraded. Genetic evidence demonstrates that the overexpression of SlCV aggravates SlPsbS protein degradation, whereas silencing of SlCSN5 and SlCV delays LNT-induced NPQ reduction and SlPsbS protein turnover. This study reveals a ubiquitin-dependent degradation pathway of chloroplast proteins co-mediated by CV and CSN5A, thereby providing a basic reference for the regulation of chloroplast protein stability under stress conditions.
